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Real-time PCR detection of the mg219 gene of unknown function of Mycoplasma genitalium in men with and without non-gonococcal urethritis and their female partners in England

¹ Health Protection Agency;

² Northampton General Hospital Trust

³ E-mail: vicki.chalker{at}hpa.org.uk

Received February 3, 2009
Accepted March 23, 2009

Real-time PCR was employed to detect a region of the mg219 gene, a gene of unknown function of Mycoplasma genitalium in clinical samples. Amplification of DNA and signal production from 15 other species of human mycoplasmas and 14 other bacteria and viruses did not occur. Using a panel of 208 genital and rectal samples the sensitivity when compared to the modified mgpa gene (encoding the major surface protein MgPa) real-time PCR assay was found to be 100 % and the specificity of the assay 99.5 % with PPV of 80 % and NPV of 100 %. The mg219 gene was found to be in all strains of M. genitalium and was highly conserved. Mycoplasma genitalium was detected in 3.9 % (11/280, 95 % CI 2.1-6.9) of all male specimens, 7.7 % (10/130 95 % CI 4.1-13.7) in patients with non-gonococcal urethritis and 0.7% (1/150 95 % CI <0.01-4.1) in patients without urethritis. The presence of M. genitalium was significantly associated with non-gonococcal urethritis (p = <0.01, 95 % Cl 0.88-0.98) and non-chlamydial-non-gonococcal urethritis (p = 0.0005; 95 % Cl 0.84-0.97).