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Published online ahead of print on 5 June 2009 as doi:10.1099/jmm.0.009811-0
Journal of Medical Microbiology 2009;58:874.

J Med Microbiol (2009), DOI: 10.1099/jmm.0.009811-0
© 2009 Society for General Microbiology
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Evaluation of new selective culture media and a rapidfluorescence in situ hybridisation (FISH) assay for identification of Clostridium difficile from stool samples

Kathrin Bloedt1, Melanie Riecker1, Sven Poppert2 and Nele Wellinghausen3,4

1 Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Germany;

2 Bernhard-Nocht-Institute for Tropical Medicine;

3 Dr. Gaertner & Partner Laboratory

4 E-mail: nele.wellinghausen{at}labor-gaertner.de

Received January 23, 2009
Accepted March 10, 2009

Two new agars, the Clostridium difficile-selective agar according to Brazier (Oxoid) and the C. difficile-selective agar (BD), were compared to cycloserine-cefoxitin-fructose agar regarding their sensitivity for culture of C. difficile from stool samples. A C. difficile-specific FISH assay was developed facilitating rapid and reliable identification of cultured isolates.







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