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Published online ahead of print on 4 June 2009 as doi:10.1099/jmm.0.009159-0
Journal of Medical Microbiology 2009;58:863.

J Med Microbiol (2009), DOI: 10.1099/jmm.0.009159-0
© 2009 Society for General Microbiology
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Tissue Microarray and Immunohistochemistry as Tools for Evaluation of Antibodies against Chlamydia-like Bacteria

Nicole Borel1, Nicola Casson2, José M. Entenza3, Carmen Kaiser1, Andreas Pospischil1 and Gilbert Greub2,4

1 Institute of Veterinary Pathology, University of Zurich, Zurich, Switzerland;

2 Institute of Microbiology, University of lausanne, Lausanne, Switzerland;

3 Dpt of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland

4 E-mail: gilbert.greub{at}chuv.ch

Received January 8, 2009
Accepted March 6, 2009

Tissue microarray technology was used to establish immunohistochemistry protocols and to determine the specificity of new antisera against various Chlamydia-like bacteria for their future use on formalin-fixed and paraffin-embedded tissues. Antisera exhibited strong reactivity against autologous antigen and closely related heterologous antigen but no cross-reactivity with distantly related species.






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