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This Article Full Text (Papers in Press[PDF]) All Versions of this Article: jmm.0.008623-0v1 58/7/945    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Ichikawa, K. Articles by Ogawa, K. PubMed PubMed Citation Articles by Ichikawa, K. Articles by Ogawa, K. Agricola Articles by Ichikawa, K. Articles by Ogawa, K.

Characterization of Mycobacterium avium clinical isolates in Japan using subspecies-specific insertion sequences, and identification of a new insertion sequence, ISMav6

¹ Department of Microbiology, Faculty of Pharmacy, Meijo University;

² Center of National University Hospital for Infection Control, Nagoya University Hospital;

³ Department of Pharmacy, National Hospital Organization, Nagoya Medical Center;

⁴ National Hospital Organization, Higashinagoya National Hospital;

⁵ National Hospital Organization, Higashinagoya National Hospital,

⁶ E-mail: ogawak{at}toumei.hosp.go.jp

Received December 9, 2008
Accepted March 20, 2009

Clinical isolates of M. avium (n=81) from patients with pulmonary infections who did not have human immunodeficiency virus (HIV) and isolates (n=33) from HIV-positive patients were subjected to genetic analysis by PCR detection of three M. avium-specific insertion sequences (IS901, IS1245, and IS1311), and nucleotide sequencing of the heat shock protein 65 (hsp65) gene. All clinical isolates were identified as M. avium subspecies hominissuis by sequence analysis of hsp65. Compared with clinical isolates of M. avium reported elsewhere, IS1245 was found less frequently in Japanese isolates (96/114 isolates, 84%) and IS901 was detected more frequently (76/114 isolates, 67%). One isolate was found to lack IS1311, which has not been reported previously for M. avium subsp. hominissuis. Nucleotide sequence analysis of the PCR products for IS901 revealed that all clinical isolates had the same new insertion sequence designated as ISMav6, which has 60 point mutations compared with the nucleotide sequence of the original IS901. These results suggest that M. avium subsp. hominissuis with ISMav6 is prevalent in Japan. ISMav6 may have implications for the virulence of M. avium and contribute to an increase of M. avium infections in this country.