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This Article Full Text (Papers in Press[PDF]) All Versions of this Article: jmm.0.007484-0v1 58/7/900    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Lee, A. S Articles by Gilbert, G. L PubMed PubMed Citation Articles by Lee, A. S Articles by Gilbert, G. L Agricola Articles by Lee, A. S Articles by Gilbert, G. L

Identification of nontuberculous mycobacteria: utility of the GenoType Mycobacterium CM/AS assay compared to HPLC and 16S rDNA sequencing

¹ University of Geneva Hospitals;

² Westmead Hospital

³ E-mail: l.gilbert{at}usyd.edu.au

Received October 22, 2008
Accepted March 27, 2009

Nontuberculous mycobacteria causing clinical disease have become increasingly common and more diverse. A new reverse line probe assay, GenoType Mycobacterium (Hain Lifescience, Germany) was evaluated for the identification of a broad range of nontuberculous mycobacteria. It was compared with phenotypic (high-performance liquid chromatography) and molecular (DNA probes, in-house real-time multiplex species-specific PCR, 16S rDNA PCR and sequencing) identification techniques, which together provided the reference 'gold standard'. A total of 131 clinical isolates belonging to 31 Mycobacterium species and 19 controls, including 5 non-Mycobacterium species, were used. Concordant results between GenoType Mycobacterium and the reference identification were obtained in 119 of 131 (90.8%) clinical isolates. Identification of M. abscessus and M. lentiflavum by the assay was problematic. The GenoType Mycobacterium assay enables rapid identification of a broad range of potentially clinically significant Mycobacterium species but some of them require further testing to differentiate or confirm ambiguous results.