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This Article Full Text (Papers in Press[PDF]) All Versions of this Article: jmm.0.006825-0v1 58/7/845    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Dong, Z. W. Articles by Li, M. Y. PubMed PubMed Citation Articles by Dong, Z. W. Articles by Li, M. Y. Agricola Articles by Dong, Z. W. Articles by Li, M. Y.

Immune effects against influenza A virus and a novel DNA vaccine with co-expression of haemagglutinin and neuraminidase genes

¹ West China School of Preclinical and Forensic Medicine, Sichuan University;

² State Key Laboratory of Oral Diseases, Sichuan University

³ E-mail: zxllmyus{at}yahoo.com

Received September 23, 2008
Accepted March 8, 2009

The high variability of influenza virus brings the difficulties to control and prevent from influenza, thus seeking a promising approach is a hot subject. Haemagglutinin (HA) and neuraminidase (NA) are major surface antigens of the influenza virus and provide effective protection against the lethal challenges with this virus. We constructed a DNA vaccine (pHA-IRES2-NA) with a co-expression of both HA and NA and compared its protective efficacy and immunogenic ability with that of a single-expression of either HA or NA, and the mixture of two single-expressions. Our findings showed that both HA and NA proteins expressed by pHA-IRES2-NA could be detected in vivo and vitro. The protections of DNA vaccines were evaluated by the serum antibody titers, residual lung virus titers, and survival rates of the mice. In murine model, immunization of pHA-IRES2-NA generated significant anti-HA and NA antibody, increased the percent of CD8+ cells and IFN--producing CD8+ cells and ratio of Th1/Th2, which was comparable to the effects of immunization with HA or NA DNA alone and the mixture of HA and NA DNA. All the mice inoculated by pHA-IRES2-NA resisted to the lethal challenge by homologous influenza virus and survived in accompany with low lung virus titer. In addition, previous studies reported the co-expression allowed higher-frequency transduction compared to co-transduction of separated vector systems encoding different genes. The novel co-expressing HA and NA DNA vaccine is a successful alternative in place of the use with the mixture of purified HA and NA proteins or HA and NA DNA.