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Distribution of fim3 and flaA TTGE sequence types amongst isolates of Bordetella bronchiseptica from different host animals

ANGELA SHINA, C. ANTHONY HART, MATTHEW D. STENTON, SUSAN DAWSON^*,, CHRISTINE M. MCCRACKEN, SARAH H. BINNS, ROSALIND M. GASKELL and CRAIG WINSTANLEY

Departments of Medical Microbiology and Genito-Urinary Medicine, *Veterinary Pathology and Veterinary Clinical Science and Animal Husbandry, University of Liverpool, PO Box 147, Liverpool L69 3GA

Corresponding author: Dr C. Winstanley (e-mail: C.Winstanley{at}liv.ac.uk).

Received 3 Dec. 2001; revised version received 1 Feb. 2002; accepted 27 Feb. 2002.

Abstract

Isolates of Bordetella bronchiseptica associated with different animal hosts were analysed by fim3 and flaA genotyping by temporal temperature gradient gel electrophoresis (TTGE). All the isolates from cats (n=138), dogs (n=42) and pigs (n=13) could be assigned to one of two fim3 and one of three flaA TTGE sequence types, respectively. Two avian isolates and a marmoset isolate exhibited novel fim3 sequence types. Dominant but different TTGE sequence types were apparent in isolates from dogs and pigs for both fim3 (87.5% and 100%, respectively, of isolates were the dominant type) and flaA (95% and 92%, respectively, of isolates were the dominant type). There was a wider distribution of TTGE sequence types amongst cat isolates. As both fimbriae and flagella have been implicated in adherence of bordetellae to host cells, sequence variations in fimbrial proteins and FlaA may have a role to play in host preferences.