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PCR ribotyping of clinically important Clostridium difficile strains from Hungary

Institute of Clinical Microbiology, Faculty of Medicine, University of Szeged, 6725 Szeged, Hungary and *PHLS Anaerobe Reference Unit, Department of Medical Microbiology and Public Health Laboratory, University of Wales College of Medicine, Cardiff CF14 4XN

Corresponding author: Professor E. Nagy (e-mail: nagye{at}mlab.szote.u-szeged.hu).

Received 20 Feb. 2001; revised version accepted 11 June 2001.

Abstract

Isolates of Clostridium difficile from different hospital wards at the University Hospital of Szeged in Hungary were typed by PCR amplification of rRNA intergenic spacer regions (PCR ribotyping). A total of 15 different ribotypes was detected among the 65 isolates tested. The predominant type, PCR ribotype 087, accounted for 39% of all isolates, in contrast with an international typing study where ribotype 001 was the most common. Two non-toxigenic C. difficile strains were found to exhibit the same pattern, which was distinct from those of all the ribotypes described previously, suggesting that this is a new type.

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