HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by KABIR, S. Search for Related Content PubMed PubMed Citation Articles by KABIR, S. Agricola Articles by KABIR, S.

Detection of Helicobacter pylori in faeces by culture, PCR and enzyme immunoassay

Academic Research and Information Management, 117 36 Stockholm, Sweden

Corresponding author: Dr S. Kabir (e-mail: skabir43{at}hotmail.com).

Received 4 Dec. 2000; revised version accepted 18 June 2001.

Abstract

Various techniques such as culture, PCR and enzyme immunoassay have been used to detect Helicobacter pylori infection in human faecal specimens. Attempts to culture H. pylori have had limited success as the bacterium exists predominantly in a non-culturable (coccoid) form in the faeces. Several PCR protocols, differing from each other in the choice of genomic targets and primers, have been used to detect H. pylori infection. Substances in faeces that inhibit PCR have been removed by various pre-PCR steps such as filtration through a polypropylene membrane, biochemical separation by column chromatography and isolation of H. pylori with immunomagnetic beads, the former two techniques yielding results with a high degree of sensitivity and specificity. An enzyme immunoassay based on the detection of H. pylori antigen in faeces has become a convenient tool for the pre-treatment diagnosis of the infection. The stool antigen assay is convenient, especially for children, as it involves neither surgery nor the discomfort associated with the urea breath test. However, its applicability in monitoring eradication therapy has been controversial, as the assay can detect dead or partially degraded bacteria long after actual eradication, thus giving false positive results.

This article has been cited by other articles:

				M. Sugimoto, J.-Y. Wu, S. Abudayyeh, J. Hoffman, H. Brahem, K. Al-Khatib, Y. Yamaoka, and D. Y. Graham Unreliability of Results of PCR Detection of Helicobacter pylori in Clinical or Environmental Samples J. Clin. Microbiol., March 1, 2009; 47(3): 738 - 742. [Abstract] [Full Text] [PDF]

				M. Shahamat, M. Alavi, J. E. M. Watts, J. M. Gonzalez, K. R. Sowers, D. W. Maeder, and F. T. Robb Development of Two PCR-Based Techniques for Detecting Helical and Coccoid Forms of Helicobacter pylori J. Clin. Microbiol., August 1, 2004; 42(8): 3613 - 3619. [Abstract] [Full Text] [PDF]

				A. M. Attallah, H. Ismail, G. G. Ibrahim, M. Abdel-Raouf, A. M. El-Waseef, and M. Abdel-Wahab Use of a Novel Enzyme Immunoassay Based on Detection of Circulating Antigen in Serum for Diagnosis of Helicobacter pylori Infection Clin. Vaccine Immunol., July 1, 2004; 11(4): 775 - 779. [Abstract] [Full Text]

				B. L. Ng, S. H. Quak, M. Aw, K. T. Goh, and B. Ho Immune Responses to Differentiated Forms of Helicobacter pylori in Children with Epigastric Pain Clin. Vaccine Immunol., September 1, 2003; 10(5): 866 - 869. [Abstract] [Full Text] [PDF]

				C. G. Harper, M. T. Whary, Y. Feng, H. L. Rhinehart, R. S. Wells, S. Xu, N. S. Taylor, and J. G. Fox Comparison of Diagnostic Techniques for Helicobacter cetorum Infection in Wild Atlantic Bottlenose Dolphins (Tursiops truncatus) J. Clin. Microbiol., July 1, 2003; 41(7): 2842 - 2848. [Abstract] [Full Text] [PDF]

				S Koletzko, N Konstantopoulos, D Bosman, A Feydt-Schmidt, A van der Ende, N Kalach, J Raymond, and H Russmann Evaluation of a novel monoclonal enzyme immunoassay for detection of Helicobacter pylori antigen in stool from children Gut, June 1, 2003; 52(6): 804 - 806. [Abstract] [Full Text]