Novel synergistic antibiofilm combinations for salvage of infected catheters, by M. Venkatesh, L. Rong, I. Raad and J. Versalovic
Journal of Medical Microbiology vol. 58, part 7, pp. 936 - 944
Supplementary Tables [PDF file] (42 KB)
Supplementary Table S1
CIs for the drug combinations against S. epidermidis biofilms.
Supplementary Table S2
CIs for the drug combinations against C. albicans biofilms.
Supplementary Figures
Supplementary Fig. S1
Viability of monomicrobial S. epidermidis biofilm sonicates after exposure to EDTA, NAC, ethanol and TLF. Biofilms of S. epidermidis strains (ATCC 55133, H100 and S101) grown in 96-well microtitre plates were exposed to EDTA and NAC at 16 mg ml-1, TLF at 8 mg ml-1, ethanol at 12.5% or growth media (control). At 0, 24, 48 and 72 h, cells from selected wells were sonicated and plated as serial dilutions. log10 c.f.u. ml-1 values are presented as the mean ± SEM. Viability of S. epidermidis ATCC 55133 (a), H100 (b) and S101 (c) was significantly decreased by EDTA and NAC (*P<0.05, two-way ANOVA). Ethanol and TLF were less consistent in reducing viability. Strain S101 was susceptible to all agents at the time points tested. [PDF file] (30 KB)
Supplementary Fig. S2
Viability of monomicrobial C. albicans biofilm sonicates after exposure to EDTA, NAC, ethanol and TLF. Biofilms of C. albicans strains (ATCC 32354 and ATCC MYA 4441) grown in 96-well microtitre plates were exposed to EDTA and NAC at 16 mg ml-1, TLF at 8 mg ml-1, ethanol at 12.5% or growth media (control). At 0, 24, 48 and 72 h, cells from selected wells were sonicated and plated as serial dilutions. log10 c.f.u. ml-1 values are presented as the mean ± SEM. EDTA, NAC and ethanol significantly reduced viability of both strains of C. albicans at 24, 48 and 72 h (*P<0.05, two-way ANOVA). TLF did not consistently reduce viability in either strain of C. albicans. [PDF file] (28 KB)
