Comparative analysis of BI/NAP1/027 hypervirulent strains reveals novel toxin B-encoding gene (tcdB) sequences, by R. A. Stabler, L. F. Dawson, L. T. H. Phua and B. W. Wren
Journal of Medical Microbiology vol. 57, part 6, pp. 771 - 775
Supplementary Table S1
Primers used for amplification and sequencing of tcdB regions e–g. [PDF file] (43 KB)
Supplementary Fig. S1
Multiple nucleotide sequence alignment of the tcdB gene for region e (a), region f (b) and region g (c). The following strains were compared for each region: CD630, BI-1, BI-2, BI-3, BI-9, BI-10, BI-11, BI-13 and BI-14. The primer sequences for amplification of the respective regions are underlined. [PDF file] (35 KB)
Supplementary Fig. S2
Multiple sequence alignment of TcdB region e (a), region f (b) and region g (c). BI-1 to BI-3 represent historic non-epidemic fluoroquinolone-sensitive strains, BI-9 to BI-14 represent fluoroquinolone-resistant epidemic strains, BI-9 clustered in the HA1 clade and BI-14 was a hypervirulent clade outlier. [PDF file] (35 KB)
