Molecular analysis of the Penicillium marneffei glyceraldehyde-3-phosphate dehydrogenase-encoding gene (gpdA) and differential expression of gpdA and the isocitrate lyase-encoding gene (acuD) upon internalization by murine macrophages, by S. Thirach, C. R. Cooper, Jr and N. Vanittanakom
Journal of Medical Microbiology vol. 57, part 11, pp. 1322 - 1328
Supplementary Fig. S1
Nucleotide and deduced amino acid sequences of the gpdA gene from P. marneffei. The G1–G4 oligonucleotides are marked by arrows. Nucleotides in upper-case letters indicate the exons, while lower-case letters indicate the introns. Nucleotides in bold italics represent the conserved 5' and 3' consensus splice sequences of each intron. The internal splice (lariat) sequences are indicated by boxes around the nucleotides. Start and stop codons are underlined. The deduced amino acid sequence is shown above the nucleotide sequence (single letter code). The sites putatively related to the inorganic phosphate binding are indicated by boxes around the amino acids. Amino acids putatively related to the NAD+ binding are shown in bold italics. The substrate-binding site is marked with a bracket. Amino acids potentially associated with catalysis are shown in bold. Circles mark the residues related to the binding to the phosphate from the substrate. Asterisks indicate the putative phosphorylation sites. [PDF file] (325 KB)
