J Med Microbiol 55 (2006), 1755-1756; DOI: 10.1099/jmm.0.46812-0
© 2006 Society for General Microbiology
ISSN 1473-5644
Interaction of Vibrio cholerae O139 with an intestinal parasite, Entamoeba histolytica
Vibhu Jain,
Mitesh Dongre and
Saumya Raychaudhuri
Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India
Correspondence
Saumya Raychaudhuri
(saumya{at}imtech.res.in or
saumya{at}rocketmail.com)
Vibrio cholerae is the causative agent of cholera, a form of diarrhoea, which continues to rage and remains a major public health problem in the developing world. The organism has the capacity to survive in diverse estuarine environments, as well as in the human host. Recent studies have suggested that interaction with a freshwater amoeba, Acanthamoeba castellanii, could be one possible mode of survival in the aquatic environment (Abd et al., 2005; Thom et al., 1992). It was also shown that V. cholerae could replicate intracellularly in A. castellanii. This prompted us to study its interaction with a parasitic amoeba, Entamoeba histolytica.
E. histolytica, the causative agent of amoebic colitis and amoebic liver abscess, is the second most common cause of death from parasitic disease worldwide (Stanley, 2003). In their natural environment, trophozoites of E. histolytica live in the colon of the human intestine together with the resident microflora, which under normal conditions is usually composed of a complex mixture of mainly anaerobic or microaerophilic bacteria (Mirelman, 1987). In order to examine the interaction of V. cholerae with the trophozoites of E. histolytica, a gentamicin assay was employed, as described previously (Venkataraman et al., 1997). In this assay, E. histolytica HM-1 : IMSS trophozoites (a kind gift from Professor Sudha Bhattacharyya, JawaharLal Nehru University, New Delhi, India) were suspended in serum-free TYI-S-33 medium at a concentration of 105 amoebae ml–1. Trophozoites were incubated in triplicate in 24-well tissue culture plates (Falcon). Subsequently, V. cholerae O139, strain SG24 (a kind gift from Dr Ranjan Nandy, National Institute of Cholera and Enteric Research, Calcutta, India), was added to a final concentration of 107 cells ml–1. The samples were incubated at 36 °C for 1 h, followed by the addition of 200 µg gentamicin ml–1 for 2 h to kill the extracellular bacteria. After gentamicin treatment, the trophozoites were washed three times with PBS by centrifuging at 280 g for 7 min. After washing, trophozoites were counted with trypan blue to check viability and lysed by syringe passage in the presence of 0.01 % Triton X-100. Dilutions were plated on Luria–Bertani agar plates for colony enumeration, which was found to be 5.53x103±0.18x103. The data suggested the presence of intracellular bacteria within the trophozoites of E. histolytica, with the trophozoites providing a protective barrier for V. cholerae against the effect of gentamicin.
To investigate the intracellular localization of the bacteria further, we carried out transmission electron microscopy of infected trophozoites. Microscopic pictures of trophozoites 1 h after infection revealed that V. cholerae O139 cells were localized intracellularly in the vacuoles (Fig. 1
).
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Fig. 1. (a) Electron micrograph of a thin section of E. histolytica trophozoites incubated for 1 h at 36 °C with V. cholerae SG24. (b) Vacuole magnification. Thin sections were prepared after epoxy-resin embedding. Bacteria were found inside the vacuoles (arrow). Bars, 500 nm (a) and 200 nm (b).
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Taken together, these findings suggest that V. cholerae O139 cells are recognized by the trophozoites of E. histolytica and are localized primarily in the vacuoles. Previously, various surface molecules of V. cholerae have been shown to be involved in diverse functions. For example, the extensively studied type 4 pilus, the toxin-coregulated pilus, is necessary for colonization of the mammalian intestine (Thelin & Taylor, 1996). Similarly, a mannose-sensitive haemagglutinin (MSHA) was found to promote the adherence of V. cholerae to zooplankton (Chiavelli et al., 2001). Besides promoting adherence to biotic surfaces, MSHA is also important for biofilm formation on abiotic (borosilicate glass) surfaces (Watnick et al., 1999). Currently, the cell-surface molecules of V. cholerae that mediate its interaction with the trophozoites of E. histolytica are not known. Additional studies are necessary to determine this. Furthermore, association of V. cholerae with free-living as well as parasitic amoebae may serve as a model for further exploration of the biology of the bacterium. This is the first report describing the interaction of V. cholerae with the human intestinal parasitic protozoan E. histolytica.
REFERENCES
Abd, H., Weintraub, A. & Sandstrom, G. (2005). Intracellular survival and replication of Vibrio cholerae O139 in aquatic free-living amoebae. Environ Microbiol 7, 1003–1008.[CrossRef][Medline]
Chiavelli, D. A., Marsh, J. W. & Taylor, R. K. (2001). The mannose-sensitive hemagglutinin of Vibrio cholerae promotes adherence to zooplankton. Appl Environ Microbiol 67, 3220–3225.[Abstract/Free Full Text]
Mirelman, D. (1987). Ameba–bacterium relationship in amebiasis. Microbiol Rev 51, 272–284.[Free Full Text]
Stanley, S. L., Jr (2003). Amoebiasis. Lancet 361, 1025–1034.[CrossRef][Medline]
Thelin, K. H. & Taylor, R. K. (1996). Toxin-coregulated pilus, but not mannose-sensitive hemagglutinin, is required for colonization by Vibrio cholerae O1 El Tor biotype and O139 strains. Infect Immun 64, 2853–2856.[Abstract]
Thom, S., Warhurst, D. & Drasar, B. S. (1992). Association of Vibrio cholerae with fresh water amoebae. J Med Microbiol 36, 303–306.[Abstract/Free Full Text]
Venkataraman, C., Haack, B. J., Bondada, S. & Abu Kwaik, Y. (1997). Identification of a Gal/GalNAc lectin in the protozoan Hartmannella vermiformis as a potential receptor for attachment and invasion by the Legionnaires' disease bacterium. J Exp Med 186, 537–547.[Abstract/Free Full Text]
Watnick, P. I., Fullner, K. J. & Kolter, R. (1999). A role for the mannose-sensitive hemagglutinin in biofilm formation by Vibrio cholerae El Tor. J Bacteriol 181, 3606–3609.[Abstract/Free Full Text]
