Genetic and phenotypic analysis of biofilm phenotypic variation in multiple Staphylococcus epidermidis isolates, by L. D. Handke, K. M. Conlon, S. R. Slater, S. Elbaruni, F. Fitzpatrick, H. Humphreys, W. P. Giles, M. E. Rupp, P. D. Fey and J. P. O’Gara
Journal of Medical Microbiology vol. 53, part 5, pp. 367 - 374
Supplementary Fig. A. Representative colonial forms as observed on CRA. Distinct phenotypes, termed crusty (shown at A), intermediate (B) and smooth (C) were detected after incubation as described in Methods. A biofilm assay was conducted on a representative colony from each colony phenotype. Results in triplicate are shown in panel D.
Supplementary Fig. B. Percentages of crusty, intermediate and smooth colony phenotypes observed on CRA after 1 and 5 days incubation of one crusty (SE5 Cr), two intermediates (SE5 Int-1 and SE5 Int-2) and one smooth (SE5 Sm) isolates.
Supplementary Fig. C. Alignments of segments of icaA and icaD sequence data obtained from SE5, SE5 PV2, SE5 PV3 and SE5 PV10.
Supplementary Fig. D. Enrichment assay as performed on smooth phenotypic variants SE5 PV2, SE5 PV3 and SE5 PV10. Reversion values, indicating percentages of colonies that were biofilm-positive as assessed by colony phenotype on CRA, were plotted for each day of the study. Overall, 17 broth passages were performed.
Supplementary Table. Biofilm production by S. epidermidis strain SE5 and phenotypic variant isolates
[PDF of Table and Figs B–D]
