Inability of outer-surface protein C (OspC)-primed mice to elicit a protective anamnestic immune response to a tick-transmitted challenge of Borrelia burgdorferi, by R. D. Gilmore, Jr, R. M. Bacon, A. M. Carpio, J. Piesman, M. C. Dolan & M. L. Mbow
Journal of Medical Microbiology vol. 52, part 7, pp. 551 - 556
Supplementary Figure. Confocal microscopy of Borrelia burgdorferi in the salivary glands of a tick that was removed at 72 h of feeding on the mouse that was protected from infection. B. burgdorferi was double-stained as described in the text. (a) The left panel shows B. burgdorferi (stained green) within the salivary glands under a fluorescein filter. The right panel shows the same field under a Texas red filter. B. burgdorferi that expresses OspC would be observed as red and is not apparent. (b) A different field from the salivary glands shown in (a), indicating the presence of an OspC-expressing B. burgdorferi cell. The left panel is viewed with the fluorescein filter. The right panel shows the same field with the Texas red filter.
