Molecular analysis of VcfQ protein involved in Vibrio cholerae type IV pilus biogenesis
J Med Microbiol
Miyazato et al. 52 (4): 283.
Western blots
Western blotting analysis
Molecular analysis of VcfQ protein involved in Vibrio cholerae type IV pilus biogenesis, by T. Miyazato, C. Toma, N. Nakasone, K. Yamamoto & M. Iwanaga
Journal of Medical Microbiology vol. 52, part 4, pp. 283 - 288
Fig. S1. Western blotting analyses using anti-VcfQT antiserum (1<2>:<2>100). (a) Immunological cross-reaction of VcfQ and MshQ. Whole-cell lysate of Escherichia coli harbouring pCTQ20 (lane1) or pQ28-4 (lane 2) are shown. (b) Localization of VcfQ in Vibrio cholerae 88UDT119. Lanes: 1, cytosol; 2, periplasm; 3, inner membrane; 4, outer membrane.
Fig. S2. Major pilin expression and extracellular pili detection. Whole-cell lysate (lanes 1, 3 and 5) and crude pili (lanes 2, 4 and 6) of the wild-type (88UDT119; lanes 1 and 2), the vcfQ mutant (WM4; lanes 3 and 4) and the complemented strain (50-2; lane 5 and 6) were prepared from 16 h cultured cells. Western blotting analysis using anti-VcfA antiserum was performed on these whole-cell lysates and crude pili. M, Molecular mass markers.
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