Title of supplement
Application of terminal RFLP analysis to characterize oral bacterial flora in saliva of healthy subjects and patients with periodontitis, by M. Sakamoto, Y. Takeuchi, M. Umeda, I. Ishikawa & Y. Benno
Journal of Medical Microbiology vol. 52, part 1, pp. 79 - 89
Supplementary Fig. 1. T-RFLP patterns of 16S rDNAs from saliva digested with HhaI (A) and MspI (B). 16S rDNAs were amplified with universal primers 27F and 1492R. Samples: (H) periodontally healthy subject and (P) patient with periodontitis. The minimum and maximum values of the ordinate of each T-RFLP pattern are 0 and 300 fluorescence units, respectively.
T-RFLP patterns 1A+B
Supplementary Fig. 2. T-RFLP patterns of 16S rDNAs from saliva digested with HhaI (A) and MspI (B). 16S rDNAs were amplified with universal primer 27F and the Spirochaeta-selective reverse primer. Samples: (H) periodontally healthy subject and (P) patient with periodontitis. The minimum and maximum values of the ordinate of each T-RFLP pattern are 0 and 2000 fluorescence units, respectively.
T-RFLP patterns 2A+B
