| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
REVIEW ARTICLE |
Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, Duncan Building, Daulby Street, Liverpool L69 3GA, UK
Corresponding author: Dr C. Winstanley (e.mail: C.Winstanley{at}liverpool.ac.uk).
Received 2 Aug. 2001; revised version received 19 Dec. 2001; accepted 17 Jan. 2002.
Abstract
Comparison of DNA from virulent strains of bacterial pathogens with DNA from less virulent or avirulent close relatives allows the identification of those genomic regions that are present only in virulent strains. Such regions are often associated with pathogenicity islands (PIs) and their characterisation can lead to a greater understanding of the pathogenesis of infectious diseases. There is now a large database of bacterial genomic sequences that provides useful reference information with which to compare the genomes of strains that exhibit variations in virulence or host preferences. Subtractive hybridisation (SH) and its sister method, suppression subtractive hybridisation (SSH), are techniques designed to identify those regions present in one genome but absent from another. The application of these techniques has led to the identification of PIs, mobile genetic elements and variations in virulence gene expression in a range of bacterial pathogens.
This article has been cited by other articles:
|
|
 |
|
  J. E. Afset, E. Anderssen, G. Bruant, J. Harel, L. Wieler, and K. Bergh Phylogenetic Backgrounds and Virulence Profiles of Atypical Enteropathogenic Escherichia coli Strains from a Case-Control Study Using Multilocus Sequence Typing and DNA Microarray Analysis J. Clin. Microbiol., July 1, 2008; 46(7): 2280 - 2290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Alavi, S. Sanjari, F. Durand, C. Brin, C. Manceau, and S. Poussier Assessment of the Genetic Diversity of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans as a Basis To Identify Putative Pathogenicity Genes and a Type III Secretion System of the SPI-1 Family by Multiple Suppression Subtractive Hybridizations Appl. Envir. Microbiol., May 15, 2008; 74(10): 3295 - 3301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Reynaud, D. Saulnier, D. Mazel, C. Goarant, and F. Le Roux Correlation between Detection of a Plasmid and High-Level Virulence of Vibrio nigripulchritudo, a Pathogen of the Shrimp Litopenaeus stylirostris Appl. Envir. Microbiol., May 15, 2008; 74(10): 3038 - 3047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. Molins-Schneekloth, J. T. Belisle, and J. M. Petersen Genomic Markers for Differentiation of Francisella tularensis subsp. tularensis A.I and A.II Strains Appl. Envir. Microbiol., January 1, 2008; 74(1): 336 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Dordet-Frisoni, G. Dorchies, C. De Araujo, R. Talon, and S. Leroy Genomic Diversity in Staphylococcus xylosus Appl. Envir. Microbiol., November 15, 2007; 73(22): 7199 - 7209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Li, C. Ryder, M. Mandal, F. Ahmed, P. Azadi, D. S. Snyder, R. D. Pechous, T. Zahrt, and T. J. Inzana Attenuation and protective efficacy of an O-antigen-deficient mutant of Francisella tularensis LVS Microbiology, September 1, 2007; 153(9): 3141 - 3153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. Osorio, S. Juiz-Rio, and M. L. Lemos A siderophore biosynthesis gene cluster from the fish pathogen Photobacterium damselae subsp. piscicida is structurally and functionally related to the Yersinia high-pathogenicity island. Microbiology, November 1, 2006; 152(Pt 11): 3327 - 3341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. H. M. Smart, F. W. Scott, E. A. Wright, M. J. Walshaw, C. A. Hart, T. L. Pitt, and C. Winstanley Development of a diagnostic test for the Midlands 1 cystic fibrosis epidemic strain of Pseudomonas aeruginosa. J. Med. Microbiol., August 1, 2006; 55(Pt 8): 1085 - 1091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. M. Smart, M. J. Walshaw, C. A. Hart, and C. Winstanley Use of suppression subtractive hybridization to examine the accessory genome of the Liverpool cystic fibrosis epidemic strain of Pseudomonas aeruginosa J. Med. Microbiol., June 1, 2006; 55(6): 677 - 688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Duangsonk, D. Gal, M. Mayo, C. A. Hart, B. J. Currie, and C. Winstanley Use of a Variable Amplicon Typing Scheme Reveals Considerable Variation in the Accessory Genomes of Isolates of Burkholderia pseudomallei J. Clin. Microbiol., April 1, 2006; 44(4): 1323 - 1334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Chen, S. J. Savarino, and M. M. Venkatesan Subtractive hybridization and optical mapping of the enterotoxigenic Escherichia coli H10407 chromosome: isolation of unique sequences and demonstration of significant similarity to the chromosome of E. coli K-12. Microbiology, April 1, 2006; 152(Pt 4): 1041 - 1054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. W. Whitby, T. M. VanWagoner, A. A. Taylor, T. W. Seale, D. J. Morton, J. J. LiPuma, and T. L. Stull Identification of an RTX determinant of Burkholderia cenocepacia J2315 by subtractive hybridization J. Med. Microbiol., January 1, 2006; 55(1): 11 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhang, U. Melcher, L. Zhou, F. Z. Najar, B. A. Roe, and J. Fletcher Genomic Comparison of Plant Pathogenic and Nonpathogenic Serratia marcescens Strains by Suppressive Subtractive Hybridization Appl. Envir. Microbiol., December 1, 2005; 71(12): 7716 - 7723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. van Bergen, G. Simons, L. van der Graaf-van Bloois, J. P. van Putten, J. Rombout, I. Wesley, and J. A Wagenaar Amplified fragment length polymorphism based identification of genetic markers and novel PCR assay for differentiation of Campylobacter fetus subspecies J. Med. Microbiol., December 1, 2005; 54(12): 1217 - 1224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Tennant, N. A. Skinner, A. Joe, and R. M. Robins-Browne Homologues of Insecticidal Toxin Complex Genes in Yersinia enterocolitica Biotype 1A and Their Contribution to Virulence Infect. Immun., October 1, 2005; 73(10): 6860 - 6867. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Bernier and P. A. Sokol Use of Suppression-Subtractive Hybridization To Identify Genes in the Burkholderia cepacia Complex That Are Unique to Burkholderia cenocepacia J. Bacteriol., August 1, 2005; 187(15): 5278 - 5291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Juiz-Rio, C. R. Osorio, V. de Lorenzo, and M. L. Lemos Subtractive hybridization reveals a high genetic diversity in the fish pathogen Photobacterium damselae subsp. piscicida: evidence of a SXT-like element Microbiology, August 1, 2005; 151(8): 2659 - 2669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Saxena, Y. Li, and P. W. Caufield Identification of Unique Bacterial Gene Segments from Streptococcus mutans with Potential Relevance to Dental Caries by Subtraction DNA Hybridization J. Clin. Microbiol., July 1, 2005; 43(7): 3508 - 3511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Marenda, E. Sagne, F. Poumarat, and C. Citti Suppression subtractive hybridization as a basis to assess Mycoplasma agalactiae and Mycoplasma bovis genomic diversity and species-specific sequences Microbiology, February 1, 2005; 151(2): 475 - 489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. N. Parsons, S. Panagea, C. H. M. Smart, M. J. Walshaw, C. A. Hart, and C. Winstanley Use of Subtractive Hybridization To Identify a Diagnostic Probe for a Cystic Fibrosis Epidemic Strain of Pseudomonas aeruginosa J. Clin. Microbiol., December 1, 2002; 40(12): 4607 - 4611. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | J MED MICROBIOL | MICROBIOLOGY | J GEN VIROL | ALL SGM JOURNALS |
