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J. Med. Microbiol. -- Vol. 51 (2002), 221-224
© 2002 Society for General Microbiology
ISSN 0022-2615

MYCOLOGY

Comparison of Candida ID medium with Sabouraud-chloramphenicol agar for the isolation of yeasts from clinical haematology surveillance specimens

W.J. OLVER, J. STAFFORD, P. CHEETHAM and T.C. BOSWELL

Department of Microbiology and Public Health Laboratory, City Hospital, Hucknall Road, Nottingham NG5 1PB

Corresponding author: Dr W. J. Olver (e-mail: mrzwo@ gwmail.nottingham.ac.uk).

Received 29 May 2001; revised version accepted 4 Sept. 2001.

Abstract

Candida ID is a new chromogenic medium for the identification of yeasts from clinical specimens. C. albicans produces blue pigmentation, whereas pink pigmentation is produced by C. tropicalis, C. lusitaniae, C. guilliermondii and C. kefyr; other Candida species appear white. In this study, 240 clinical samples (throat swabs and stool samples) from haematology patients were inoculated on to Candida ID and Sabouraud-chloramphenicol agar in parallel, yielding a total of 105 yeasts; the media had overall detection rates of 85.7% and 86.7% respectively. The sensitivity of Candida ID for identification of C. albicans by blue pigmentation was 52.9% at 24 h and 94.1% at 48 h. Specificity of the blue pigmentation was 100% at 48 h. Two strains of C. tropicalis were identified, one produced pink pigmentation at 72 h, the other strain did not produce any pigmentation after 5 days. Candida ID was superior in detecting mixtures of yeasts compared with Sabouraud-chloramphenicol agar. Candida ID is a suitable primary isolation medium for yeasts from clinical specimens, providing rapid direct identification of C. albicans and enhanced detection of mixtures.






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