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J. Med. Microbiol. -- Vol. 50 (2001), 663-666
© 2001 Society for General Microbiology
ISSN 0022-2615

DIAGNOSTIC MICROBIOLOGY

Evaluation of three methods to measure anti-Brucella IgM antibodies and interference of IgA in the interpretation of mercaptan-based tests

T. MARRODAN*, R. NENOVA-POLIAKOVA*,||, M. RUBIO*, J. ARIZA{dagger}, E. CLAVIJO{ddagger}, H.L. SMITS§ and R. DIAZ*

*Departamento de Microbiología, Servicio de Microbiología Clínica, Universidad de Navarra, Pamplona Spain, {dagger}Servicio de Enfermedades Infecciosas, Hospital de Bellvitge, Barcelona, Spain, {ddagger}Servicio de Microbiología, Hospital Virgen de la Victoria, Málaga, Spain and §Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands

Corresponding author: Dr R. Diaz (e-mail: rdiaz{at}unav.es). ||Present address: National Center of Infectious and Parasitic Diseases, 44A General Stoletov BLD, BG-1233, Sofia, Bulgaria.

Received 28 July 2000; revised version accepted 4 Jan. 2001.

Abstract

The results of a dipstick assay for the detection of immunoglobulin M (IgM) to Brucella smooth lipopolysaccharide (S-LPS) correlated with those of an enzyme-linked immunosorbent assay (ELISA) for IgM and of the serum agglutination test (SAT) performed with and without dithiothreitol. Two sera which were dithiothreitol-sensitive and were dipstick negative were shown to contain specific IgA. The dipstick assay is recommended as a simple method for detecting specific IgM antibodies in acute-phase brucellosis patients.




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[Abstract] [Full Text] [PDF]


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