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J. Med. Microbiol. -- Vol. 50 (2001), 339-344
© 2001 Society for General Microbiology
ISSN 0022-2615


BACTERIAL CHARACTERISATION

Demonstration of the Rb1 lipopolysaccharide core structure in Salmonella strains with the monoclonal antibody M105

LUCIELLE P. MANSFIELD and S.J. FORSYTHE

Department of Life Sciences, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS

Corresponding author: Dr S.J. Forsythe (e-mail: stephen. forsythe{at}ntu.ac.uk).

Received 10 April 2000; revised version accepted 12 Sept. 2000.

Abstract

The lipopolysaccharide (LPS) from 42 strains representing 19 Salmonella serogroups was differentiated into characteristic ladder-like profiles by SDS-PAGE analysis. The core-specific antibody M105 (Ra, Rb1 and Rb2) was used in an immunoblot assay of SDS-PAGE-separated LPS molecules. The M105 antibody bound to the R-type LPS of 18 of the 20 Salmonella strains tested. The results demonstrate that S. enterica serotype Godesberg, S. Adelaide (one of two strains), S. Milwaukee, S. Niarembe, S. Bere and S. Arizonae (serogroup 63) have an atypical LPS core structure which is Rb1 type.




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Innate ImmunityHome page
C. Whitfield, N. Kaniuk, and E. Frirdich
Molecular insights into the assembly and diversity of the outer core oligosaccharide in lipopolysaccharides from Escherichia coli and Salmonella
Innate Immunity, August 1, 2003; 9(4): 244 - 249.
[Abstract] [PDF]




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