J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by FEODOROVA, V.A.
Right arrow Articles by DEVDARIANI, Z.L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by FEODOROVA, V.A.
Right arrow Articles by DEVDARIANI, Z.L.
Agricola
Right arrow Articles by FEODOROVA, V.A.
Right arrow Articles by DEVDARIANI, Z.L.
J. Med. Microbiol. -- Vol. 50 (2001), 969-978
© 2001 Society for General Microbiology
ISSN 0022-2615

MICROBIAL PATHOGENICITY

New genes involved in Yersinia pestis fraction I biosynthesis

V.A. FEODOROVA and Z.L. DEVDARIANI

Laboratory of Hybridomas, Russia State Antiplague Research Institute ‘Microbe', Saratov, Russia

Corresponding author: Dr V. A. Feodorova (e-mail: microbe{at}san.ru)

Received 9 Oct. 2000; revised version accepted 11 April 2001.

Abstract

Antigenic and immunochemical properties of Yersinia pestis fraction I (FI) preparations extracted by different methods were studied with polyclonal and monoclonal antibodies. The existence of mature FI in a form of a complex antigen whose subunits have different genetic control was demonstrated. Galactolipid was shown, with caf1 product, to be the second species-specific component of the FI complex molecule and is probably encoded by chromosomal genes. It, like caf1 product, was expressed in higher quantities at 37°C than at 28°C. Among FI subunits there were at least two proteins of 28 ± 2 kDa and 43 ± 2 kDa which were not specific for Y. pestis but were found also in all Yersinia spp. and some other bacteria. These proteins were synthesised independently of the incubation temperature (4°–40°C) and are possibly encoded chromosomally but outside the caf operon and galactolipid-encoding genes. Both proteins together with galactolipid comprise an envelope antigen found in pFra- or plasmidless Y. pestis strains. Organisation of Y. pestis FI (mature capsular antigen) in the form of a complex of the envelope antigen and the caf1 product is discussed.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2001 Society for General Microbiology.