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A potential role for tumour necrosis factor- in synergy between porcine respiratory coronavirus and bacterial lipopolysaccharide in the induction of respiratory disease in pigs

Laboratory of Veterinary Virology, Faculty of Veterinary Medicine, University of Ghent, B-9820 Merelbeke, Belgium

Corresponding author: Dr K. Van Reeth (e-mail: kristien.vanreeth{at}rug.ac.be).

Received 10 June 1999; revised version accepted 2 Dec. 1999.

Abstract

This study examined whether exposure of pigs to both porcine respiratory coronavirus (PRCV) and bacterial lipopolysaccharide (LPS) can potentiate respiratory disease and lung secretion of tumour necrosis factor- (TNF-) and interleukin-1 (IL-1). Caesarian-derived colostrum-deprived pigs were inoculated intratracheally with PRCV, with LPS from Escherichia coli O111:B4 (20 µg/kg), or with a combination of the two, and killed at set times after inoculation. Clinical signs, virus replication and (histo)pathological changes in the lungs, percentage of neutrophils and bioactive TNF- and IL-1 in broncho-alveolar lavage (BAL) fluids were examined. The effects of separate virus or LPS inoculations were subclinical and failed to induce high and sustained cytokine levels. In a preliminary study, pigs were inoculated with PRCV and then with LPS 24 h later and killed sequentially. Severe respiratory disease and significantly enhanced TNF- titres (208–3601 U/ml versus 40–89 U/ml after LPS only) were seen during the first 12 h after LPS inoculation. IL-1 levels (106–1631 U/ml versus 28–654 U/ml after LPS only) were also increased, but persisted for longer after clinical recovery than TNF-. In a second study, pigs were inoculated with PRCV and subsequently with LPS at various time intervals ranging from 0 to 24 h, and killed 5 h after inoculation with LPS. A time interval of at least 12 h between inoculations was necessary for prominent respiratory signs to develop. Production of TNF-, but not IL-1, was also dependent on the time interval between inoculations and was tightly correlated with disease. Lung neutrophil infiltration and pathological changes were comparable after combined PRCV-LPS and single LPS inoculations, and were not associated with disease. These data show that exposure to high endotoxin concentrations in swine buildings can precipitate respiratory disease in PRCV-infected pigs, and that TNF- is probably an important mediator of these effects. This is the first in-vivo demonstration of synergy between respiratory viruses and LPS.

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