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The role of SEF14 and SEF17 fimbriae in the adherence of Salmonella enterica serotype Enteritidis to inanimate surfaces

Department of Bacteriology, Veterinary Laboratories Agency (Weybridge), Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB and *Food Microbiology Research Unit, Public Health Laboratory Service, Church Lane, Heavitree, Exeter EX2 5AD

Corresponding author: Professor M. J. Woodward (e-mail: mwoodward.cvl.wood{at}gtnet.gov.uk).

Received 8 June 1999; revised version received 1 Oct. 1999; accepted 9 Nov. 1999.

Abstract

To gain an understanding of the role of fimbriae and flagella in the adherence of Salmonella enterica serotype Enteritidis to inanimate surfaces, the extent of adherence of viable wild-type strains to a polystyrene microtitration plate was determined by a crystal violet staining assay. Elaboration of surface antigens by adherent bacteria was assayed by fimbriae- and flagella-specific ELISAs. Wild-type Enteritidis strains adhered well at 37°C and 25°C when grown in microtitration wells in Colonisation Factor Antigen broth, but not in other media tested. At 37°C, adherent bacteria elaborated copious quantities of SEF14 fimbrial antigen, whereas at 25°C adherent bacteria elaborated copious quantities of SEF17 fimbrial antigen. Non-fimbriate and non-flagellate knock-out mutant strains were also assessed in the adherence assay. Mutant strains unable to elaborate SEF14 and SEF17 fimbriae adhered poorly at 37°C and 25°C, respectively, but adherence was not abolished. Non-motile mutant strains showed reduced adherence whilst type-1, PEF and LPF fimbriae appeared not to contribute to adherence in this assay. These data indicate that SEF17 and SEF14 fimbriae mediate bacterial cell aggregation on inanimate surfaces under appropriate growth conditions.

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