HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by BROWN, N. F. Articles by BEACHAM, I. R. Search for Related Content PubMed PubMed Citation Articles by BROWN, N. F. Articles by BEACHAM, I. R. Agricola Articles by BROWN, N. F. Articles by BEACHAM, I. R.

Cloning and analysis of genomic differences unique to Burkholderia pseudomallei by comparison with B. thailandensis

School of Health Science, Griffith University, Gold Coast Campus, Southport, Queensland 4217, Australia

Corresponding author: Dr I.R. Beacham (e-mail: I.beacham{at}mailbox.gu.edu.au).

Received 4 Jan. 2000; revised version accepted 30 March 2000.

Abstract

Melioidosis is an infectious disease caused by Burkholderia pseudomallei. Genomic subtractive hybridisation was performed with the closely related avirulent species B. thailandensis to identify virulence genes of B. pseudomallei. The subtractive hybridisation products were highly specific for B. pseudomallei. Sequence analysis revealed a number of putative virulence factors, as well as apparently novel sequences of unknown function. The subtracted library contained DNA regions of relatively low G+C mol% content, which were distributed throughout the B. pseudomallei genome. The distribution of subtracted sequences amongst a collection of 22 B. pseudomallei isolates was found to be variable, with the exception of three strains which almost universally lacked the subtracted sequences. These three strains also differed in that they were highly haemolytic, indicating a possible separate virotype.

This article has been cited by other articles:

				L. R. Triplett, Y. Zhao, and G. W. Sundin Genetic Differences between Blight-Causing Erwinia Species with Differing Host Specificities, Identified by Suppression Subtractive Hybridization Appl. Envir. Microbiol., November 1, 2006; 72(11): 7359 - 7364. [Abstract] [Full Text] [PDF]

				J. A. Boddey, C. P. Flegg, C. J. Day, I. R. Beacham, and I. R. Peak Temperature-Regulated Microcolony Formation by Burkholderia pseudomallei Requires pilA and Enhances Association with Cultured Human Cells Infect. Immun., September 1, 2006; 74(9): 5374 - 5381. [Abstract] [Full Text] [PDF]

				A. E. Essex-Lopresti, J. A. Boddey, R. Thomas, M. P. Smith, M. G. Hartley, T. Atkins, N. F. Brown, C. H. Tsang, I. R. A. Peak, J. Hill, et al. A Type IV Pilin, PilA, Contributes to Adherence of Burkholderia pseudomallei and Virulence In Vivo Infect. Immun., February 1, 2005; 73(2): 1260 - 1264. [Abstract] [Full Text] [PDF]

				M. T. G. Holden, R. W. Titball, S. J. Peacock, A. M. Cerdeno-Tarraga, T. Atkins, L. C. Crossman, T. Pitt, C. Churcher, K. Mungall, S. D. Bentley, et al. From the Cover: Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei PNAS, September 28, 2004; 101(39): 14240 - 14245. [Abstract] [Full Text] [PDF]

				D. DeShazer Genomic Diversity of Burkholderia pseudomallei Clinical Isolates: Subtractive Hybridization Reveals a Burkholderia mallei-Specific Prophage in B. pseudomallei 1026b J. Bacteriol., June 15, 2004; 186(12): 3938 - 3950. [Abstract] [Full Text] [PDF]

				C. WINSTANLEY Spot the difference: applications of subtractive hybridisation to the study of bacterial pathogens J. Med. Microbiol., June 1, 2002; 51(6): 459 - 467. [Abstract] [Full Text] [PDF]

				N. F. Brown, J. A. Boddey, C. P. Flegg, and I. R. Beacham Adherence of Burkholderia pseudomallei Cells to Cultured Human Epithelial Cell Lines Is Regulated by Growth Temperature Infect. Immun., February 1, 2002; 70(2): 974 - 980. [Abstract] [Full Text] [PDF]