J Med Microbiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Published online ahead of print on 24 September 2009 as doi:10.1099/jmm.0.013870-0
J Med Microbiol (2009), DOI: 10.1099/jmm.0.013870-0
© 2009 Society for General Microbiology
This Article
Right arrow Full Text (Papers in Press[PDF])
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by De Baere, T.
Right arrow Articles by Vaneechoutte, M.
PubMed
Right arrow PubMed Citation
Right arrow Articles by De Baere, T.
Right arrow Articles by Vaneechoutte, M.
Agricola
Right arrow Articles by De Baere, T.
Right arrow Articles by Vaneechoutte, M.

Evaluation of ITS2-RFLP-analysis for the identification of dermatophytes (Fungi)

Thierry De Baere1, Richard Summerbell2, Bart Theelen2, Teun Boekhout2 and Mario Vaneechoutte1,3

1 Laboratory for Bacteriology Research, Ghent University Hospital, Ghent University, Gent, Belgium;

2 CBS Fungal Diversity Centre, Utrecht, The Netherlands

3 E-mail: mario.vaneechoutte{at}ugent.be

Received June 29, 2009
Accepted September 18, 2009

A total of 95 isolates, belonging to 33 species of 5 dermatophyte genera, i.e. Arthroderma (15 species), Chrysosporium (2), Epidermophyton (1), Microsporum (3) and Trichophyton (12), were studied with ITS2-PCR-RFLP analysis (ITS2-RFLP), consisting of amplification of the ITS2 region, restriction digestion with BstUI (CG/CG) and restriction fragment length determination by capillary electrophoresis on an ABI Prism 310 (Applied BioSystems). ITS2-RFLP-analysis proved to be most useful for identification of species of the genera Arthroderma, Chrysosporium and Epidermophyton, but could not distinguish between several Trichophyton species. The identification results are in correspondence with established and recent taxonomical insights of the dermatophytes, e.g. because highly related species also had closely related and sometimes difficult-to-discriminate ITS2-RFLP patterns. In some cases, several ITS2-RFLP-groups could be distinguished within species, again mostly in correspondence with the taxonomic delineations of subspecies and/or genomovars, confirming the relevance of ITS2-RFLP-analysis as an identification technique and a useful taxonomic approach.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2009 Society for General Microbiology.