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Vibrio cholerae O1 Ogawa detoxified lipopolysaccharide structures as inducers of cytokines and oxidative species in macrophages

¹ Slovak Academy of Sciences, Institute of Chemistry, Centre of Excellence GLYCOMED,;

² Slovak Academy of Sciences, Institute of Virology

³ E-mail: ema.paulovicova{at}savba.sk

Received June 16, 2009
Accepted October 7, 2009

Multi-drug resistance to several strains of Vibrio cholerae supported the anti-cholera vaccine developmental attempts via various sub-cellular moieties. In order to examine immunological efficacy of dLPS derived saccharidic immunogens, ex vivo activation of mouse peritoneal macrophage was investigated. Immunomodulatory effect was evaluated via induction of pro-inflammatory cytokines TNF-, IL-1 , IL-6 and acceleration of nitric oxide and reactive oxygen species (NOS/ROS). Immunologically active structures triggered mouse peritoneal macrophages to cytokine secretion and NOS/ROS release, even at very low concentrations of 12.5µg/mL. It could be assumed that O-specific polysaccharidic moiety is more immunologically efficient than glycolipidic one, probably due to KDO position. Present study revealed effective structure-immunomodulating relationships of detoxified LPS–derived moieties, desirable in sub-cellular anti-cholera vaccine design.