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Evaluation of PCR MP method for intra-species differentiation of Trichophyton rubrum and Trichophyton interdigitale

¹ Technical University of Gdansk;

² University of Lodz, Department of Genetics of Microorganisms;

³ Technical University of Gdansk, Department of Microbiology;

⁴ Medical University of Lodz, Department of Dermatology and Venerology

⁵ E-mail: justyna_leibner{at}o2.pl

Received June 8, 2009
Accepted November 3, 2009

In order to identify the sources of infection caused by dermatophytes as well as the pathogen transmission pathway there is the need to determine the methods that allow for the deep genetic differentiation of the strains within the genus. In this work for the first time PCR MP technique based on the ligation of adaptors and the difference in the melting temperatures of DNA restriction fragments was used for intraspecies genotyping of dermatophytes. Clinical isolates and reference strains of dermatophytes isolated from skin, toe and finger nails were used for this study. PCR MP (PCR Melting Profiles) and RAPD method has been applied for typing 11 isolates of T. rubrum, 40 isolates of T. interdigitale and 14 isolates of M. canis. We distinguished 5 types (containing 1 subtype) characteristic for T. rubrum, and 7 types characteristic for T. interdigitale with the PCR MP technique. Analysis conducted with RAPD method revealed 5 types for T. rubrum and 4 types for T. interdigitale isolates. No differentiation was observed in the case of M. canis isolates. These results demonstrate that PCR MP is a reliable method for the differentiation of T. rubrum and T. interdigitale strain and yields at least similar discriminatory power as the RAPD method.