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Published online ahead of print on 27 August 2009 as doi:10.1099/jmm.0.013235-0
Journal of Medical Microbiology 2009;58:1542.

J Med Microbiol (2009), DOI: 10.1099/jmm.0.013235-0
© 2009 Society for General Microbiology
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Burkholderia cenocepacia O antigen lipopolysaccharide prevents phagocytosis by macrophages and adhesion to epithelial cells

M. Soledad Saldias, Ximena Ortega and Miguel A Valvano1

University of Western Ontario

1 E-mail: miguel.valvano{at}schulich.uwo.ca

Received May 29, 2009
Accepted August 20, 2009

Chronic respiratory infections by the Burkholderia cepacia complex (Bcc) are of great concern to patients with cystic fibrosis. Bcc isolates may survive intracellularly within amoebae, respiratory epithelial cells, and macrophages. The molecular mechanisms facilitating colonization and pathogenesis remain unclear. Given the importance of bacterial adhesion to host surfaces in microbial pathogenesis, we investigated the role of the O antigen lipopolysaccharide in the interaction of B. cenocepacia, a member of the Bcc, with macrophages and epithelial cells. Our results demonstrate that the O antigen modulates phagocytosis but does not affect intracellular survival of B. cenocepacia. Internalization of strains that lack O antigen was significantly increased compared to that of their isogenic smooth counterparts. However, no differences between rough and smooth strains were found in their ability to delay phagosomal maturation. We also found that the O antigen interfered with the ability of B. cenocepacia to adhere to bronchial epithelial cells, suggesting that this polysaccharide may mask one or more bacterial surface adhesins.






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