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This Article Full Text (Papers in Press[PDF]) All Versions of this Article: jmm.0.012856-0v1 58/12/1611    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Chitambar, S. D. Articles by Chhabra, P. PubMed PubMed Citation Articles by Chitambar, S. D. Articles by Chhabra, P. Agricola Articles by Chitambar, S. D. Articles by Chhabra, P.

Molecular characterization of a rare G1P[19] rotavirus strain from India: evidence of reassortment between human and porcine rotavirus strains

National Institute of Virology

¹ E-mail: chitambars{at}hotmail.com

Received May 14, 2009
Accepted August 9, 2009

This study pertains to the characterization of a human rotavirus strain (NIV929892) with a rare specificity of G1P[19]. Three structural (VP4, VP6 and VP7) and one nonstructural (NSP4) genes of the NIV929893 strain were subjected to RT- PCR for amplification of entire coding regions. All of the amplicons were sequenced to carry out phylogenetic analysis. The complete amino acid sequences of VP7 and VP4 genes showed clustering of the VP7 gene with G1 strains of human origin and the VP4 gene with P[19] strains of porcine origin, respectively. The two viral proteins, VP6 and NSP4, described earlier as genetically linked proteins, were shown to be SGII and genotype B, however, of human and porcine origins, respectively. The findings of this study provide an evidence of reassortment between VP7/VP6 genes of human, and VP4/NSP4 genes of porcine species and an independent segregation of VP6 and NSP4 genes in a group A human rotavirus strain with G1P[19] specificity.