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Identification of Burkholderia cepacia complex (Bcc) bacteria with a lipopolysaccharide specific monoclonal antibody

¹ University of Nevada Reno;

² Universtiy of Michigan Medical School

³ E-mail: daucoin{at}medicine.nevada.edu

Received April 29, 2009
Accepted September 1, 2009

The genus Burkholderia includes many bacteria that cause serious human infections. As is the case with other Gram-negative bacteria, Burkholderia species produce lipopolysaccharides (LPS) which is an abundant component of the bacterial cell surface. Burkholderia cepacia complex (Bcc) bacteria (including at least 17 separate species) produce LPS structures that are quite different. In an attempt to determine the degree of LPS epitope variation among Bcc species, we produced a monoclonal antibody (mAb), designated 5D8, specific for the LPS of B. cepacia. Western blot analysis determined that mAb 5D8 was able to produce the classic 'ladder pattern' when used to probe B. cepacia and B. anthina lysates, although 5D8 did not produce this pattern with the other seven Bcc species tested. mAb 5D8 reacted with varying intensity to most but not all of the additional B. cepacia and B. anthina strains tested. Therefore, there seems to be significant epitope variation among Bcc LPS both between and within species. Additionally, mAb 5D8 reacted with a proteinase K-sensitive 22 kD antigen in all Bcc strains and also in a strain of Burkholderia pseudomallei.