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This Article Full Text (Papers in Press[PDF]) All Versions of this Article: jmm.0.010090-0v1 58/12/1576    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Perera, K. Articles by Murray, A. PubMed PubMed Citation Articles by Perera, K. Articles by Murray, A. Agricola Articles by Perera, K. Articles by Murray, A.

Development of an indirect ELISA for the detection of serum IgG antibodies against region IV of phase 1-flagellin of Salmonella enterica serovar Brandenburg in sheep

¹ AgResearch, National Centre for Biosecurity and Infectious Disease-Wallaceville, New Zealand;

² IVABS, Massey University, Palmerston North, New Zealand

³ E-mail: kalyani.perera{at}agresearch.co.nz

Received February 6, 2009
Accepted August 13, 2009

Region IV of phase-1 flagellin (FliC) of Salmonella enterica subsp. enterica serovar Brandenburg was expressed in Escherichia coli and purified by nickel chelate affinity chromatography. The purified recombinant protein was evaluated for its suitability as an antigen in an indirect ELISA for the detection of antibodies in sheep sera. A cut-off value of 0.1 was calculated using 80 serum samples collected from sheep with no previous history of S. Brandenburg. In the present study, we show the results of ELISA with field sera collected from 81 sheep naturally infected with S. Brandenburg. The assay was able to detect antibodies belonging to IgG class with a sensitivity of 93.8%. Thus, indirect ELISA might be a suitable screening tool for serological monitoring of sheep flocks infected with S. Brandenburg.