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This Article Full Text (Papers in Press[PDF]) All Versions of this Article: jmm.0.007609-0v1 58/12/1607    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Pereira-Silveira, C. Articles by Dolande, M. PubMed PubMed Citation Articles by Pereira-Silveira, C. Articles by Dolande, M. Agricola Articles by Pereira-Silveira, C. Articles by Dolande, M.

Minimum inhibitory and minimum fungicidal concentrations of amphotericin B, itraconazole, posaconazole and terbinafine in Sporothrix schenckii

¹ Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;

² Unitat de Recerca en Malalties Infeccioses i Micologia, Institut Municipal d'Investigacio Medica;

³ Mycology Department, Natonal Institute of Hygiene Rafael Rangel, Bolivarian Republic of Venezuela

⁴ E-mail: carolina.silveira{at}ufrgs.br

Received November 3, 2008
Accepted August 7, 2009

The in vitro susceptibility of 62 isolates of Sporothrix schenckii in its mycelial form, most of them from Latin-American countries (Peru, Venezuela, Brazil, Uruguay) and Spain, to amphotericin B, itraconazole, posaconazole and terbinafine was determined by measuring the minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC), employing the standardised CLSI method.Terbinafine was the most active drug, showing the lowest geometric mean (GM) MIC and MFC between the five country/groups tested. Itraconazole and posaconazole showed almost the same activity against all the strains tested, except for the isolate from Uruguay against itraconazole that showed the highest GM (10.68 mg L- 1). Amphotericin B showed the widest MIC susceptibility range (0.03 - 16.0 mg L-1), but this drug was less active showed the highest GM MIC (1.40 mg L- 1) between the isolates.MFCs were always high, from five to 20 times higher than the MICs, but the lowest GM and range values were found for terbinafine. Itraconazole and posaconazole showed the highest GM MFC. MFC may be a better predictor of therapeutic response than MIC, especially in immunosuppressed patients and, if considered, making the use of itraconazole and posaconazole an inappropriate treatment. There are some differences in susceptibility according to the geographical precedence of the isolates, it being lower for terbinafine in Venezuelan strains (P = 0.066) for MIC and higher for posaconazole in Peruvian strains (P = 0.02) for MFC. It seems that geographical origin could be important for the appropriate treatment, even to the identification of the species of the S. schenckii complex and the disease manifestation.