The Journal of Medical Microbiology, Vol 9, Issue 4 371-377, Copyright © 1976 by Society for General Microbiology
Purity of staphylococcal delta-haemolysin obtained by three different procedures
S. H. Lee, T. Sarauskas, E. S. Hoy and R. U. Haque
Three methods at present available for the purification of staphylococcal
delta-haemolysin were compared as to the purity and identify of the product
obtained. None yielded a pure preparation of delta-haemolysin; one of the
three preparations did not contain demonstrable delta-haemolysin when
tested electrophoretically, but it contained deoxyribonuclease,
penicillinase, phosphatase and alpha-haemolysin. The second preparation had
delta-haemolysin activity and was free of alpha-haemolysin, but it
contained lipase, egg-yolk factor, esterase, deoxyribonuclease,
penicillinase, phosphatase and hyaluronidase. The third preparation
contained all of the products mentioned above, except phosphatase, and it
also contained alpha-haemolysin, staphylokinase, lysozyme and caseinase.
These findings are discussed with special reference to the requirement for
criteria of purity in work with staphylococcal products.
