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The Journal of Medical Microbiology, Vol 8, Issue 1 173-176, Copyright © 1975 by Society for General Microbiology

JOURNAL ARTICLE

A simple single-step immunoimmobilisation method for the detection of Salmonella in the presence of large numbers of other bacteria

B. Mohit, R. Aly and L. D. Bourgeois

A single-step disk immunoimmobilisation method for the detection of motile salmonellae is described. It combines in a single step the use of the motility of these bacteria in soft agar, their selective growth on Salmonella-Shigella media, and their immobilisation by polyvalent H antiserum. The method is shown to be 10 to 10,000 times more senstitive than a standard method for detection of Salmonella under the experimental conditons used. The time required for detection is shortened to 24-48 h.




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