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This Article Full Text Full Text (PDF) Supplementary Data All Versions of this Article: jmm.0.011767-0v1 58/9/1236    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Google Scholar Articles by Huang, H. Articles by Wang, Y.-D. PubMed PubMed Citation Articles by Huang, H. Articles by Wang, Y.-D. Agricola Articles by Huang, H. Articles by Wang, Y.-D.

Salmonella expressing a T-cell epitope from Sendai virus are able to induce anti-infection immunity

¹ Laboratory for Immunology and Microbiology, BioMedical Education Center, Peking University Health Science Center, Beijing, PR China

² Department of Microbiology, Peking University Health Science Center, Beijing, PR China

³ Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Canada

⁴ Department of Immunology, Peking University Health Science Center, Beijing, PR China

⁵ Genomics Research Center, Harbin Medical University, Harbin 150081, PR China

Correspondence
Shu-Lin Liu
slliu{at}ucalgary.ca
Yue-Dan Wang
wangyuedan{at}bjmu.edu.cn

Received April 9, 2009
Accepted June 2, 2009

Bacterial fimbriae can accept foreign peptides and display them on the cell surface. A highly efficient gene replacement method was used to generate peptide vaccines based on Salmonella enterica subsp. enterica serovar Typhimurium LT2. DNA encoding an epitope from Sendai virus, SV9 (Sendai virus nucleoprotein peptide 324–332, FAPGNYPAL), which is known to induce cytotoxic T lymphocytes, was incorporated into the gene encoding AgfA (the major subunit protein of thin aggregative fimbriae of Salmonella) by replacing an equal length DNA segment. To improve cytotoxic T lymphocyte recognition, both termini of the peptide were flanked by double alanine (AA) or arginine (RR) residues. Western blotting and immunofluorescence microscopy using AgfA-specific antiserum verified the expression of chimeric AgfA; expression was also proved by a Congo red binding assay. Oral immunizations of C57BL/6 mice with the four strains induced an epitope-specific T-cell response (detected by enzyme-linked immunosorbent spot assay). When the mice were challenged with the Sendai virus, the magnitude of the infection was significantly reduced in the immunized groups compared with the controls. The Salmonella fimbrial display system efficiently induces a cellular immune response and anti-infection immunity in vivo, providing a new strategy for the development of efficient peptide vaccination.

A table of primer sequences is available as supplementary data with the online version of this paper.