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J Med Microbiol 58 (2009), 1219-1226; DOI: 10.1099/jmm.0.011452-0
© 2009 Society for General Microbiology
ISSN 0022-2615

Characterization of meticillin-resistant Staphylococcus aureus isolates from hospitals in KwaZulu-Natal province, Republic of South Africa

Adebayo Shittu1,2, Ulrich Nübel3, Edet Udo4, Johnson Lin2 and Sedio Gaogakwe2

1 Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria

2 School of Biochemistry, Genetics and Microbiology, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban, Republic of South Africa

3 Robert Koch Institute, 38855 Wernigerode, Germany

4 Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait

Correspondence
Adebayo Shittu
bayo_shittu{at}yahoo.com

Received March 31, 2009
Accepted May 22, 2009

Epidemiological data based on phenotypic and molecular characterization of meticillin-resistant Staphylococcus aureus (MRSA) in sub-Saharan Africa are limited. This investigation studied 61 MRSA isolates obtained from 13 health-care institutions in KwaZulu-Natal (KZN) province, South Africa, from March 2001 to August 2003. More than 80 % of the isolates were resistant to at least four classes of antibiotics and six isolates were resistant to the aminoglycoside, macrolide-lincosamide and tetracycline groups of antibiotics, heavy metals and nucleic acid-binding compounds. PFGE of SmaI-digested genomic DNA revealed seven types, designated A–G. Type A was the main pulsotype (62.3 %) and was identified in 11 of the 13 health-care institutions, suggesting that it represented a major clone in health-care institutions in KZN province. Analysis of representative members of the three major pulsotypes by spa, multilocus sequence typing and SCCmec typing revealed the types t064-ST1173-SCCmec IV and t064-ST1338-SCCmec IV (PFGE type A, single-locus and double-locus variants of ST8), t037-ST239-SCCmec III (PFGE type F) and t045-ST5-SCCmec III (PFGE type G). The combination of various typing methods provided useful information on the geographical dissemination of MRSA clones in health-care institutions in KZN province. The observation of major clones circulating in health-care facilities in KZN province indicates that adequate infection control measures are urgently needed.






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