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J Med Microbiol 58 (2009), 1173-1181; DOI: 10.1099/jmm.0.009175-0
© 2009 Society for General Microbiology
ISSN 0022-2615

Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates

Gustavo M. Cerqueira1,2, Alan J. A. McBride3, Mathieu Picardeau2, Samuel G. Ribeiro1, Ângela N. Moreira1, Viviane Morel2, Mitermayer G. Reis3, Albert I. Ko3,4 and Odir A. Dellagostin1

1 Federal University of Pelotas, Biotechnology Centre, PO Box 354, 96010-900 Pelotas, RS, Brazil

2 Institut Pasteur, Unité de Biologie des Spirochètes, 75015 Paris, France

3 Gonçalo Moniz Research Centre, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, BA, Brazil

4 Division of International Medicine and Infectious Disease, Weill Medical College of Cornell University, New York, NY, USA

Correspondence
Gustavo M. Cerqueira
gcenbiot{at}ufpel.edu.br

Received March 19, 2009
Accepted June 1, 2009

The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence of lig genes among serovars from a collection of leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited number of pathogenic serovars, the ligB gene was distributed ubiquitously among all pathogenic strains. None of the lig genes were detected among intermediate or saprophytic Leptospira species. It was also shown that, similar to the previously characterized secY gene, a short specific PCR fragment of ligB could be used to correctly identify pathogenic Leptospira species. These findings demonstrate that ligB is widely present among pathogenic strains and may be useful for their reliable identification and classification.

The GenBank/EMBL/DDBJ accession numbers for the lig gene sequences of the Leptospira strains described in this study are EU938447–EU938521.




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