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J Med Microbiol 58 (2009), 900-904; DOI: 10.1099/jmm.0.007484-0
© 2009 Society for General Microbiology
ISSN 0022-2615

Identification of non-tuberculous mycobacteria: utility of the GenoType Mycobacterium CM/AS assay compared with HPLC and 16S rRNA gene sequencing

Andie S. Lee1,{dagger}, Peter Jelfs1, Vitali Sintchenko1,2 and Gwendolyn L. Gilbert1,2

1 Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Sydney, Australia

2 Western Clinical School, University of Sydney, Sydney, Australia

Correspondence
Gwendolyn L. Gilbert
l.gilbert{at}usyd.edu.au

Received October 22, 2008
Accepted March 27, 2009

Non-tuberculous mycobacteria (NTM) causing clinical disease have become increasingly common and more diverse. A new reverse line probe assay, GenoType Mycobacterium CM/AS (Hain Lifescience), was evaluated for identification of a broad range of NTM. It was compared with phenotypic (HPLC) and molecular (DNA probes, in-house real-time multiplex species-specific PCR, 16S rRNA gene PCR and sequencing) identification techniques, which together provided the reference ‘gold standard’. A total of 131 clinical isolates belonging to 31 Mycobacterium species and 19 controls, including 5 non-Mycobacterium species, was used. Concordant results between the GenoType Mycobacterium assay and the reference identification were obtained in 119/131 clinical isolates (90.8 %). Identification of Mycobacterium abscessus and Mycobacterium lentiflavum by the assay was problematic. The GenoType Mycobacterium assay enables rapid identification of a broad range of potentially clinically significant Mycobacterium species, but some species require further testing to differentiate or confirm ambiguous results.

{dagger}Present address: Infection Control Program, University of Geneva Hospitals, Geneva, Switzerland.






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