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Escherichia coli O123 O antigen genes and polysaccharide structure are conserved in some Salmonella enterica serogroups

Clifford G. Clark^1,2, Andrew M. Kropinski³, Haralambos Parolis⁴, Christopher C. R. Grant¹, Keri M. Trout-Yakel¹, Kristyn Franklin³, Lai-King Ng^1,2, Nikolay A. Paramonov^4,, Lesley A. S. Parolis⁴, Kris Rahn³ and Helen Tabor¹

¹ Enteric Diseases Program, Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada

² Department of Medical Microbiology, 510 Medical Sciences Building, University of Manitoba, 730 William Avenue, Winnipeg, MB R3T 2N2, Canada

³ Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, ON N1G 3W4, Canada

⁴ School of Pharmaceutical Sciences, Rhodes University, Grahamstown 6140, South Africa

Correspondence
Clifford G. Clark
clifford_clark{at}phac-aspc.gc.ca

Received October 6, 2008
Accepted March 23, 2009

The serotyping of O and H antigens is an important first step in the characterization of Salmonella enterica. However, serotyping has become increasingly technically demanding and expensive to perform. We have therefore sequenced additional S. enterica O antigen gene clusters to provide information for the development of DNA-based serotyping methods. Three S. enterica isolates had O antigen gene clusters with homology to the Escherichia coli O123 O antigen region. O antigen clusters from two serogroup O58 S. enterica strains had approximately 85 % identity with the E. coli O123 O antigen region over their entire length, suggesting that these Salmonella and E. coli O antigen regions evolved from a common ancestor. The O antigen cluster of a Salmonella serogroup O41 isolate had a lower level of identity with E. coli O123 over only part of its O antigen DNA cluster sequence, suggesting a different and more complex evolution of this gene cluster than those in the O58 strains. A large part of the Salmonella O41 O antigen DNA cluster had very close identity with the O antigen cluster of an O62 strain. This region of DNA homology included the wzx and wzy genes. Therefore, molecular serotyping tests using only the O41 or O62 wzx and wzy genes would not differentiate between the two serogroups. The E. coli O123 O-antigenic polysaccharide and its repeating unit were characterized, and the chemical structure for E. coli O123 was entirely consistent with the O antigen gene cluster sequences of E. coli O123 and the Salmonella O58 isolates. An understanding of both the genetic and structural composition of Salmonella and E. coli O antigens is necessary for the development of novel molecular methods for serotyping these organisms.

Present address: Centre for Infectious Disease, Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, 4 Newark Street, London E1 2AT, UK.

The GenBank/EMBL/DDBJ accession numbers for the Salmonella enterica isolates S897 (serotype 58:l,z₁₃,z₂₈:1,5), S1669 (serotype 58:r:z₅₃,z₄₇:z₇₃) and 07-0209 (serotype 41:z₄,z₂₃:-) sequences are EU825756, EU825757 and EU825758, respectively.

A sequence alignment figure and tables of HMBC spectroscopy and NOESY data are available as supplementary material with the online version of this paper.