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J Med Microbiol 58 (2009), 874-877; DOI: 10.1099/jmm.0.009811-0
© 2009 Society for General Microbiology
ISSN 0022-2615

Evaluation of new selective culture media and a rapid fluorescence in situ hybridization assay for identification of Clostridium difficile from stool samples

Kathrin Bloedt1, Melanie Riecker1, Sven Poppert2 and Nele Wellinghausen1,3

1 Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Ulm, Germany

2 Department of Molecular Parasitology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany

3 Dr Gaertner & Partner Laboratories, Ravensburg, Germany

Correspondence
Nele Wellinghausen
nele.wellinghausen{at}labor-gaertner.de

Received January 23, 2009
Accepted March 10, 2009

Two new Clostridium difficile-selective agars, from Oxoid (according to Brazier) and from BD, were compared with cycloserine-cefoxitin-fructose agar (Oxoid) for their sensitivity of recovery of toxigenic C. difficile from stool samples. For the culture-positive samples, the sensitivities were 84.0, 42.6 and 90.4 %, respectively. In addition, a C. difficile-specific fluorescence in situ hybridization assay was developed, facilitating rapid and reliable identification of cultured isolates.






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