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J Med Microbiol 58 (2009), 863-866; DOI: 10.1099/jmm.0.009159-0
© 2009 Society for General Microbiology
ISSN 0022-2615

Tissue microarray and immunohistochemistry as tools for evaluation of antibodies against Chlamydia-like bacteria

Nicole Borel1, Nicola Casson2, José M. Entenza3, Carmen Kaiser1, Andreas Pospischil1 and Gilbert Greub2

1 Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland

2 Center for Research on Intracellular Bacteria, Institute of Microbiology, University of Lausanne, Lausanne, Switzerland

3 Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland

Correspondence
Gilbert Greub
gilbert.greub{at}chuv.ch

Received January 8, 2009
Accepted March 6, 2009

Tissue microarray technology was used to establish immunohistochemistry protocols and to determine the specificity of new antisera against various Chlamydia-like bacteria for future use on formalin-fixed and paraffin-embedded tissues. The antisera exhibited strong reactivity against autologous antigen and closely related heterologous antigen, but no cross-reactivity with distantly related species.






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