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J Med Microbiol 58 (2009), 791-797; DOI: 10.1099/jmm.0.009274-0
© 2009 Society for General Microbiology
ISSN 0022-2615

In vitro activity of carvacrol against staphylococcal preformed biofilm by liquid and vapour contact

Antonia Nostro1, Andreana Marino1, Anna Rita Blanco2, Luigina Cellini3, Mara Di Giulio3, Francesco Pizzimenti1, Andrea Sudano Roccaro2 and Giuseppe Bisignano1

1 Pharmaco-Biological Department, University of Messina, Messina, Italy

2 SIFI Pharma SpA, Catania, Italy

3 Department of Biomedical Sciences, School of Pharmacy, University ‘G. d'Annunzio’, Chieti, Italy

Correspondence
Antonia Nostro
atnostro{at}pharma.unime.it

Received January 6, 2009
Accepted March 2, 2009

Carvacrol is an important component of essential oils and recently has attracted much attention as a result of its biological properties, such as a wide spectrum of antimicrobial activity. The aim of this study was to evaluate the effect of carvacrol in liquid and vapour phase on preformed biofilms of Staphylococcus aureus and Staphylococcus epidermidis by determining biofilm biomass and cultivable cell numbers, and by using epifluorescence and scanning electron microscopy. Carvacrol was able to reduce biofilm biomass and cell viability more effectively when used with liquid contact rather than with vapour phase. The efficacy of treatment with carvacrol vapour was found to be dependent on exposure time. The predominance of red fluorescence using a LIVE/DEAD BacLight Viability kit (Molecular Probes) and the partially destroyed biofilm architecture as determined by microscopy in treated samples provided evidence for the efficacy of carvacrol. The findings of this investigation suggest a potential application for carvacrol in the inactivation of staphylococcal biofilms.

Abbreviations: TTC, 2,3,5-triphenyltetrazolium chloride.






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