J Med Microbiol International Journal of Systematic and Evolutionary Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tapsall, J.
Right arrow Articles by Whiley, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tapsall, J.
Right arrow Articles by Whiley, D.
Agricola
Right arrow Articles by Tapsall, J.
Right arrow Articles by Whiley, D.
J Med Microbiol 58 (2009), 683-687; DOI: 10.1099/jmm.0.007641-0
© 2009 Society for General Microbiology
ISSN 0022-2615

Case Report

Two cases of failed ceftriaxone treatment in pharyngeal gonorrhoea verified by molecular microbiological methods

John Tapsall1, Phillip Read2, Christopher Carmody3, Christopher Bourne2, Sanghamitra Ray1, Athena Limnios1, Theo Sloots4 and David Whiley4

1 World Health Organization Collaborating Centre for STD, Prince of Wales Hospital, Sydney, Australia

2 Sydney Sexual Health Centre, Sydney Hospital, Sydney, Australia

3 Short Street Sexual Health Clinic, St George Hospital, Sydney, Australia

4 Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital and Health Service District, Queensland, Australia

Correspondence
John Tapsall
j.tapsall{at}unsw.edu.au

Received October 30, 2008
Accepted January 16, 2009

Diagnostic, genotypic and antibiotic-resistance determinants of Neisseria gonorrhoeae were analysed by molecular methods to verify the failure of ceftriaxone treatment in two cases of pharyngeal gonorrhoea. Monoplex assays were needed to define competitive inhibition of a positive Chlamydia PCR in a duplex assay. Different penA changes were detected in the N. gonorrhoeae isolated from the two cases. These were associated with raised ceftriaxone MICs of 0.03 and 0.016 mg l–1, which may have contributed to the treatment failures in these cases.

Abbreviations: BHQ, black-hole quencher; FAM, 6-carboxyfluorescein; FVU, first void urine; IM, intramuscular; NAAT, nucleic acid amplification test; NG-MAST, Neisseria gonorrhoeae multi-antigen sequence typing.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2009 Society for General Microbiology.