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An in vitro evaluation of hydrolytic enzymes as dental plaque control agents

¹ School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester M13 9PT, UK

² Colgate–Palmolive Company, 909 River Road, Piscataway, NJ 08855, USA

Correspondence
Ruth G. Ledder
ruth.ledder{at}manchester.ac.uk

Received September 16, 2008
Accepted December 25, 2008

The plaque-control potential of commercially available amylase, lipase and protease was evaluated by observing their effects on coaggregation and on bacterial viability within various plaque microcosms. A quantitative coaggregation assay indicated that protease significantly inhibited the extent of coaggregation of Actinomyces naeslundii and Streptococcus oralis (P <0.05) and of Porphyromonas gingivalis and S. oralis. Amylase significantly (P <0.05) increased the coaggregation of A. naeslundii versus Fusobacterium nucleatum and A. naeslundii versus P. gingivalis. Concomitant challenge of constant-depth film fermenter-grown plaques with the enzymes did not result in detectable ecological perturbations (assessed by differential culture and denaturing gradient gel electrophoresis). Similar dosing and analysis of multiple Sorbarod devices did not reveal increases in bacterial dispersion which could result from disaggregation of extant plaques. A short-term hydroxyapatite colonization model was therefore used to investigate possible enzyme effects on early-stage plaque development. Whilst culture did not indicate significant reductions in adhesion or plaque accumulation, a vital visual assay revealed significantly increased aggregation frequency following enzyme exposure. In summary, although hydrolytic enzymes negatively influenced binary coaggregation, they did not cause statistically significant changes in bacterial viability within plaque microcosms. In contrast, enzyme exposure increased aggregation within extant plaques.

Abbreviations: CDFF, constant-depth film fermenter; DGGE, denaturing gradient gel electrophoresis; HA, hydroxyapatite; MSD, multiple Sorbarod devices.