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J Med Microbiol 58 (2009), 423-429; DOI: 10.1099/jmm.0.007831-0
© 2009 Society for General Microbiology
ISSN 0022-2615

An entropy-optimized multilocus approach for characterizing the strains of Anaplasma phagocytophilum infecting horses in the Czech Republic

Petr Zeman1,{dagger} and Petr Jahn2

1 Medical Laboratories, Konevova 205, 130 00 Prague 3, Czech Republic

2 Equine Clinic, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, 612 42 Brno, Czech Republic

Correspondence
Petr Zeman
zeman3{at}post.cz

Received November 10, 2008
Accepted January 5, 2009

Anaplasma phagocytophilum is a tick-borne rickettsial pathogen that has measurable genetic heterogeneity across its geographical range and reservoir spectrum. In the present study, publicly available sequences of the genes that have prevailingly been used for typing A. phagocytophilum were analysed to identify the segments giving the highest resolution with respect to the predictability of host and geographical provenances of the strains. Selected partial sequences of 16S rRNA, groL, msp4 and ank genes were then employed in a tentative multilocus typing scheme used to characterize the strains causing equine granulocytic anaplasmosis (EGA). We were able to both identify alleles characteristic for equine strains of A. phagocytophilum and distinguish two unique genetic variants infecting horses in the Czech Republic. This resolution far exceeded the discriminatory potential of any of the four sequenced genes when used singly. The two novel A. phagocytophilum variants appeared to be phylogenetically closer to the strains reported as causing human disease in Slovenia than to strains thus far isolated from other European EGA cases. A decline in the quality of recently deposited A. phagocytophilum sequences was also demonstrated.

Abbreviations: EGA, equine granulocytic anaplasmosis.

{dagger}Present address: Crop Research Institute, Drnovska 507, 161 06 Prague 6, Czech Republic.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, groSL, msp4 and ank gene sequences of A. phagocytophilum detected in horses in the Czech Republic reported in this study are EU839852, EU839853, EU839854, EU839855 and EU839856.

Figures showing the residue polymorphism analyses of A. phagocytophilum groSL, msp4 and ank sequences are available as supplementary data with the online version of this paper.






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