J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kawai, M.
Right arrow Articles by Yamagishi, J.-i.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kawai, M.
Right arrow Articles by Yamagishi, J.-i.
Agricola
Right arrow Articles by Kawai, M.
Right arrow Articles by Yamagishi, J.-i.
J Med Microbiol 58 (2009), 331-336; DOI: 10.1099/jmm.0.004184-0
© 2009 Society for General Microbiology
ISSN 0022-2615

Cell-wall thickness: possible mechanism of acriflavine resistance in meticillin-resistant Staphylococcus aureus

Mako Kawai1, Sakuo Yamada2,3, Ai Ishidoshiro4, Yoshihiro Oyamada5, Hideaki Ito6 and Jun-ichi Yamagishi1

1 Microbiological Control Laboratories, Technology Research and Development Center, Dainippon Sumitomo Pharma Co. Ltd, 5-51 Ebie 1-chome Fukushima-ku, Osaka 553-0001, Japan

2 Department of Microbiology, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama 701-0192, Japan

3 Department of Clinical Nutrition, Kawasaki University of Medical Welfare, 288 Matsushima, Kurashiki, Okayama 701-0193, Japan

4 Quality Assurance Group, Ibaraki Manufacturing Plant, Dainippon Sumitomo Pharma Co. Ltd, 3-45 1-chome Kurakakiuchi, Ibaraki, Osaka 567-0878, Japan

5 Pharmacology Research Laboratories, Dainippon Sumitomo Pharma Co. Ltd, Enoki, 33-94 Suita, Osaka 564-0053, Japan

6 Pharmacovigilance, Dainippon Sumitomo Pharma Co. Ltd, 6-8 Doshomachi 2-Chome, Chuo-ku, Osaka 541-0045, Japan

Correspondence
Jun-ichi Yamagishi
junichi-yamagishi{at}ds-pharma.co.jp

Received June 13, 2008
Accepted October 27, 2008

Acriflavine resistance in the clinical meticillin-resistant Staphylococcus aureus isolate KT24 was found not to be mediated by multidrug efflux pumps encoded by qacA/B, smr, qacE, qacG, qacH, qacJ or norA. Early uptake and accumulation of ethidium bromide in MRSA KT24 was significantly lower than that in a susceptible strain, although the efflux rates were similar. Therefore, a permeability barrier in MRSA KT24 may be the conceivable mechanism of acriflavine resistance. Interestingly, it was found that MRSA KT24 had a significantly thickened cell wall, and that cell-wall thickness increased gradually during bacterial growth. In contrast, cell size and surface area in MRSA KT24 were not different from those in the susceptible strain. Moreover, MRSA KT24 exposure to sub-MIC concentrations of acriflavine resulted in a thicker cell wall. These results indicate that cell-wall thickness may be responsible for acriflavine resistance in S. aureus.

Abbreviations: MRSA, meticillin-resistant Staphylococcus aureus.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2009 Society for General Microbiology.