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J Med Microbiol 58 (2009), 318-321; DOI: 10.1099/jmm.0.000380-0
© 2009 Society for General Microbiology
ISSN 0022-2615

Characterization of the charge variants of L2 β-lactamase in Stenotrophomonas maltophilia

Rouh-Mei Hu1, Kai-Hung Chiang2, Yi-Chih Chang2 and Tsuey-Ching Yang2

1 Department of Biotechnology and Bioinformatics, Asia University, Taichung 413, Taiwan, ROC

2 Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan, ROC

Correspondence
Tsuey-Ching Yang
tcyang{at}mail.cmu.edu.tw

Received January 14, 2008
Accepted November 27, 2008

Stenotrophomonas maltophilia KH has two acid β-lactamases with isoelectric points (pIs) of 4.6 and 5.4, and several basic β-lactamases (pIs >7.0) that produce a ladder-shaped pattern by IEF. An isogenic L2 mutant, KHL2xylE, was constructed by gene replacement. From IEF and native PAGE zymograms of strains KH and KHL2xylE, it was demonstrated that the basic β-lactamases and the acid β-lactamase with pI 5.4 are encoded by the same L2 gene and that the active types of these L2 charge variants were dependent on the buffer pH. The β-lactamase activities of these L2 charge variants in phosphate buffer at pH 7.0 and 8.0 were 1075±29 and 1114±81 U mg–1, respectively. These results indicate that L2 charge variants give S. maltophilia a better chance of adapting and surviving in response to changes in the environment.

Abbreviations: pI, isoelectric point.

The GenBank/EMBL/DDBJ accession number for the sequence of the ampR gene, intergenic region and L2 β-lactamase gene is EU032534.






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