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J Med Microbiol 58 (2009), 217-221; DOI: 10.1099/jmm.0.006874-0
© 2009 Society for General Microbiology
ISSN 0022-2615

Identification of plasmid- and integron-borne blaIMP-1 and blaIMP-10 in clinical isolates of Serratia marcescens

Zhuting Hu1 and Wei-Hua Zhao2

1 Department of Biology, The College of Liberal Arts, International Christian University, Tokyo 181-8585, Japan

2 Department of Microbiology and Immunology, Showa University School of Medicine, Tokyo 142-8555, Japan

Correspondence
Zhuting Hu
taketei154{at}gmail.com

Received September 24, 2008
Accepted October 10, 2008

The emergence of carbapenem-hydrolysing metallo-β-lactamases (MBLs) is a serious threat to the clinical utility of carbapenems. This study identified plasmid- and integron-borne blaIMP-1 and blaIMP-10 in clinical isolates of Serratia marcescens. The blaIMP-1 and blaIMP-10 gene cassettes were carried by a class 1 integron and followed by the aac(6')-IIc gene cassette. The blaIMP-1 and blaIMP-10 gene cassettes were preceded by a weak Pant promoter, TGGACA(N)17TAAGCT, and an inactive P2 promoter, TTGTTA(N)14TACAGT. These genes were easily transferred to Escherichia coli by conjugation and transformation, indicating that they are located on transferable plasmids. Due to the acquisition of blaIMP-1, the susceptibility of E. coli transconjugants to imipenem, meropenem, panipenem and biapenem decreased by 32-, 256-, 64- and 128-fold, respectively. In comparison, after gaining blaIMP-10, the susceptibility of E. coli transconjugants to the four carbapenems decreased by 64-, 2048-, 256- and 64-fold, respectively. Strains harbouring blaIMP-10 showed higher-level resistance to imipenem, meropenem and panipenem than the strains harbouring blaIMP-1, although the nucleotide sequences of the class 1 integrons carrying blaIMP-10 and blaIMP-1 were identical except for a single point mutation.

Abbreviations: AMK, amikacin; BIPM, biapenem; CAZ, ceftazidime; CS, conserved segment; GEN, gentamicin; IPM, imipenem; MBL, metallo-β-lactamase; MEPM, meropenem; PAPM, panipenem; SMA, sodium mercaptoacetic acid.




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W.-H. Zhao, G. Chen, R. Ito, and Z.-Q. Hu
Relevance of resistance levels to carbapenems and integron-borne blaIMP-1, blaIMP-7, blaIMP-10 and blaVIM-2 in clinical isolates of Pseudomonas aeruginosa
J. Med. Microbiol., August 1, 2009; 58(8): 1080 - 1085.
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