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J Med Microbiol 58 (2009), 192-195; DOI: 10.1099/jmm.0.004531-0
© 2009 Society for General Microbiology
ISSN 0022-2615

Molecular diagnosis of Aspergillus endocarditis after cardiac surgery

Parisa Badiee, Abdolvahab Alborzi, Elaheh Shakiba, Mazyar Ziyaeyan and Bahman Pourabbas

Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Correspondence
Parisa Badiee
badieep{at}yahoo.com

Received June 30, 2008
Accepted October 21, 2008

The prevalence of Aspergillus endocarditis (AE) is increasing in the hospital population. Aspergillus species contribute to approximately 25 % of all cases of fungal endocarditis. This study is a descriptive report of the use of nested PCR to detect DNA specific for Aspergillus species in serum for the diagnosis of cardiac infections. Open heart surgery was performed on patients and collected samples were examined microscopically and cultured. Ten sera in total from five patients were extracted for Aspergillus DNA and nested PCR with Aspergillus species primers was carried out. The lowest limit of detection for the PCR assay was 1 c.f.u. (ml serum) –1. The PCR was positive in three patients. Culture of valvular tissue confirmed the growth of Aspergillus fumigatus in one patient and Aspergillus niger in two patients. In this study we have demonstrated the presence of invasive aspergillosis in patients who had undergone open heart surgery and the usefulness of a molecular assay for the diagnosis of AE.

Abbreviations: AE, Aspergillus endocarditis; PVE, prosthetic valve endocarditis.






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